American Journal of Medical and Clinical Sciences. 2019; 4(1):(32-126)


Study of single-nucleotide polymorphism of phosphatase and tensin homolog in patients with hepatocellular carcinoma and cirrhosis associated with chronic hepatitis C virus

Amany Ragab Youssef, Abd Elmohsen E. Eldesoky

Abstract
Background: Phosphatase and tensin homolog (PTEN) is known as anti-oncogene that is located on chromosome 10q23.3. This gene has high mutations frequency in different human cancer. We investigated the prevalence of single-nucleotide polymorphisms (SNP) in the PTEN gene rs2299939 in patients with hepatocellular carcinoma (HCC) and cirrhosis associated with hepatitis C.
Material and Method: The study included 25 patients with HCC and 25 patients with cirrhosis. All patients had chronic viral hepatitis C genotype 4. Molecular study of SNP of PTEN rs2299939 polymorphism was carried out by restriction fragment length polymorphism and polymerase chain reaction.
Results: There was a statistically significant increase in GG genotype OR 2.2 (95% CI, 0.8–5.6) in patients with cirrhosis and in patients with HCC compared to the control group (p = 0.0001). There was a significant increase in GT genotype OR 1.9 (0.6–5.8) and TT genotype OR 0.7 (0.5–1.1) in control compared to patients with cirrhosis and with HCC associated with hepatitis C virus (HCV) (p = 0.0001). The frequency of G alleles had a statistically significant prevalence in patients compared to control (p = 0.0001) while T alleles had a statistically significant increase in control compared to the patients (p = 0.0001).
Conclusion: The GG genotype of PTEN gene rs2299939 showed a significant increase in patients with HCC and cirrhosis associated with HCV genotype 4. On the other hand, the TT and GT genotypes may be associated with the decreased risk of the development of HCC. Further studies are required to validate these findings.
Most Viewed Article
  • Evaluation of Effectiveness of Modified Pediatric Constraint-Induced Movement Therapy Performed on Children with Cerebral Palsy by a Home-Treatment Program
    Hiroshi Tanabe, Hirofumi Tanabe, Shosaku Okuda, Toshimasa Mikawa, Yoshimi Sakurai, Yuichi Takata.
    » Abstract » doi: 10.33425/2832-4226/25001

  • Risk Factors Associated with the Onset of Diabetes Mellitus in Patients with A History of Gestational Diabetes at The University Hospital of Kinshasa
    Tandu Umba A, Mbungu Mwimba R, Kahindo Muyayalo P, Mbangama Muela A, Lumaya Ambis J, Mwimba Mbungu N, Kelele Goy Sambwa, Lukunda Diansambu P, Kinkenda Nsiangangu B.
    » Abstract » doi: 10.33425/2832-4226/24025

  • Crisis of Soul II
    Julian Ungar-Sargon
    » Abstract » doi: 10.33425/2832-4226/24024

  • The Importance of Family Involvement in the Treatment of Pediatric Obsessive-Compulsive Disorder
    Eda Gorbis, Jenny C Yip, Aanya Jajoo.
    » Abstract » doi: 10.33425/2832-4226/24023

  • Double Diabetes: Insulin Resistance and Metabolic Syndrome in Sudanese Patients with Type 1 Diabetes Mellitus
    Alaa Ali Elsharief Hammad, Abdelaziz SI, Salih H. S. Hamid.
    » Abstract » doi: 10.33425/2832-4226/24022

Most Downloaded
  • Evaluation of Effectiveness of Modified Pediatric Constraint-Induced Movement Therapy Performed on Children with Cerebral Palsy by a Home-Treatment Program
    Hiroshi Tanabe, Hirofumi Tanabe, Shosaku Okuda, Toshimasa Mikawa, Yoshimi Sakurai, Yuichi Takata.
    » Abstract » doi: 10.33425/2832-4226/25001

  • Risk Factors Associated with the Onset of Diabetes Mellitus in Patients with A History of Gestational Diabetes at The University Hospital of Kinshasa
    Tandu Umba A, Mbungu Mwimba R, Kahindo Muyayalo P, Mbangama Muela A, Lumaya Ambis J, Mwimba Mbungu N, Kelele Goy Sambwa, Lukunda Diansambu P, Kinkenda Nsiangangu B.
    » Abstract » doi: 10.33425/2832-4226/24025

  • Crisis of Soul II
    Julian Ungar-Sargon
    » Abstract » doi: 10.33425/2832-4226/24024

  • The Importance of Family Involvement in the Treatment of Pediatric Obsessive-Compulsive Disorder
    Eda Gorbis, Jenny C Yip, Aanya Jajoo.
    » Abstract » doi: 10.33425/2832-4226/24023

  • Double Diabetes: Insulin Resistance and Metabolic Syndrome in Sudanese Patients with Type 1 Diabetes Mellitus
    Alaa Ali Elsharief Hammad, Abdelaziz SI, Salih H. S. Hamid.
    » Abstract » doi: 10.33425/2832-4226/24022