The transforming growth factor-beta (TGF-β) family consists of three isoforms: TGF-β1, TGF-β2, and TGF-β3, which play pivotal roles in cellular communication and regulation of biological processes, including immune response, inflammation, tissue repair, and development. Despite structural and functional similarities, each isoform exhibits unique expression profiles and cell-specific actions. TGF-β1 (Transforming Growth Factor Beta 1) is well-known for its immunosuppressive effects, particularly in promoting regulatory T-cell differentiation and controlling extracellular matrix (ECM) production. Its dysregulation is linked to fibrosis and autoimmune disorders. TGF-β2 (Transforming Growth Factor Beta 2) plays a critical role in embryonic development, neural tissue differentiation, and regeneration, with alterations contributing to conditions like Marfan syndrome and tumor metastasis. TGF-β3 (Transforming Growth Factor Beta 3) is essential for scar-free wound healing and tissue morphogenesis by minimizing excessive ECM deposition. TGF-β signaling occurs via Smad-dependent and alternative pathways, with crosstalk involving Wnt and NF-κB (Nuclear Factor Kappa B) pathways. Therapeutically, TGF-β inhibitors are under investigation for fibrosis and cancer, while TGF-β3 shows promise in scar reduction. Understanding the distinct roles and signaling mechanisms of TGF-β isoforms enhances prospects for targeted interventions in fibrosis, cancer, and regenerative medicine.